003 Real-time assessment of Krebs cycle metabolism with hyperpolarised [2-13c]pyruvate
نویسندگان
چکیده
منابع مشابه
Metabolism of [2-14C]acetate and its use in assessing hepatic Krebs cycle activity and gluconeogenesis.
To examine the fate of the carbons of acetate and to evaluate the usefulness of labeled acetate in assessing intrahepatic metabolic processes during gluconeogenesis, [2-14C]acetate, [2-14C]ethanol, and [1-14C]ethanol were infused into normal subjects fasted 60 h and given phenyl acetate. Distributions of 14C in the carbons of blood glucose and glutamate from urinary phenylacetylglutamine were d...
متن کاملThe relationship between calcium-phosphorus metabolism, the 'Krebs cycle' and steroid metabolism.
It has been shown in the course of the last few years that the number of factors playing a part in calcium-phosphorus (Ca-P) metabolism is far greater than had been known. It was found that the calcification of bones depends in the first place on the 'citric acid cycle' and on adenosine triphosphate (ATP) (oxidative phosphorylation) (Dickens, 1941; Albaum, Hirshfeld and Sobel, 1952; Dixon and P...
متن کاملThin layer chromatography of Krebs cycle acids.
A systematic appraisal of a large number of solvent systems was carried out for the separation of lactic, pyruvic and 3-hydroxybutyric acids and all of the KREBS cycle acids, except oxalosuccinic, using silica gel G and cellulose thin layer Chromatographie plates. Four solvent systems which allowed separation of various combinations of acids are described and the limits of this method of separa...
متن کاملHyperpolarised [2-C]Pyruvate Uniquely Reveals the Role of Acetylcarnitine as a Mitochondrial Substrate Buffer in the Heart
Introduction In normal hearts, Krebs cycle flux is maintained by continuous citrate synthase-mediated condensation of acetyl-CoA with oxaloacetate. In turn, acetylCoA is produced from the oxidation of fatty acids, ketone bodies or glucose, via glycolysis and the enzyme pyruvate dehydrogenase (PDH). Hyperpolarised [2C]pyruvate has been used in the isolated heart to observe the relationship betwe...
متن کاملAssessment of in vivo metabolism in failing hearts using hyperpolarised 13C magnetic resonance
Purpose The aim of this study was to monitor carbohydrate metabolism alongside cardiac structure and function, throughout HF progression. Methods Dilated cardiomyopathy (DCM) was induced in pigs (n = 4) by rapid ventricular pacing at 188 bpm for 4-5 weeks. Pigs were examined at baseline and at weekly time points throughout DCM progression. At each time point, cine MRI was used to assess cardiac...
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ژورنال
عنوان ژورنال: Heart
سال: 2010
ISSN: 1355-6037
DOI: 10.1136/hrt.2009.191049c